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Thermo Fisher purelinktm genomic dna mini kit
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Purelinktm Genomic Dna Mini Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Transnetyx metagenomics sequencing analysis
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Metagenomics Sequencing Analysis, supplied by Transnetyx, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co ultra one step cloning kit vazyme nanjing china
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Ultra One Step Cloning Kit Vazyme Nanjing China, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia mirna first strand cdna synthesis kit
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Mirna First Strand Cdna Synthesis Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genecopoeia first strand cdna synthesis kit
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
First Strand Cdna Synthesis Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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New England Biolabs onetaq one-step rt-pcr kit
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Onetaq One Step Rt Pcr Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co hiscript 1st strand cdna synthesis kit
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Hiscript 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology terminal deoxynucleotidyl transferase dutp nick end labeling kit
Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and <t>genomic</t> <t>DNA</t> was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Terminal Deoxynucleotidyl Transferase Dutp Nick End Labeling Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and genomic DNA was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Folate Carrier Deficiency Drives Differential Methylation and Enhanced Cellular Potency in the Neural Plate Border

doi: 10.3389/fcell.2022.834625

Figure Lengend Snippet: Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and genomic DNA was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.

Article Snippet: Genomic DNA from two independent replicates composed by ∼18 electropored embryos with Rfc1-MO and Control-MO each one was extracted by using PurelinkTM Genomic DNA Mini Kit (Invitrogen) following the manufacture’s instruction.

Techniques: Methylation, Sequencing, Control, Isolation, Functional Assay