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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Folate Carrier Deficiency Drives Differential Methylation and Enhanced Cellular Potency in the Neural Plate Border
doi: 10.3389/fcell.2022.834625
Figure Lengend Snippet: Global analysis of cytosine methylation by reduced representation bisulfate sequencing (RRBS) on folate carrier deficient embryos. (A) Schematic representation of loss-of-function experiments performed to generate a folate carrier deficient model. Embryos were bilaterally electroporated with Rfc1 (RFC1-MO) or control (Control) morfolinos, grown until HH7 and genomic DNA was isolated from each side for RRBS sequencing. (B) Pie chart analysis of overall cytosine methylation at the three genomic contexts (CG, CHG, and CHH) in control and RFC1-MO treated embryos. (C) Comparative analysis of methylation levels in distinct genomic functional elements displayed for CG, CHG, and CHH in control and RFC1-MO treated embryos. Motif occurrence for CG, CHG, and CHH context are shown on the insets for each graph. (D) Averages for methylation levels in functional regions for CG, CHG, and CHH in control and RFC1-MO treated embryos. Nonsignificant differences were found in all the global methylation analysis (B,C,D) between treatments. upstream2k: 2 Kb upstream of the transcription start site; downstream2k: 2 Kb downstream of the transcription end site.
Article Snippet: Genomic DNA from two independent replicates composed by ∼18 electropored embryos with Rfc1-MO and Control-MO each one was extracted by using
Techniques: Methylation, Sequencing, Control, Isolation, Functional Assay